Oxidative stress in sperm affects the epigenetic reprogramming in early embryonic development.

ABSTRACT:

Background

Reactive oxygen species (ROS)-induced oxidative stress is well known to play a major role in male infertility. Sperm are sensitive to ROS damaging effects because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. However, how oxidative DNA lesions in sperm affect early embryonic development remains elusive.

Results

Using cattle as model, we show that fertilization using sperm exposed to oxidative stress caused a major developmental arrest at the time of embryonic genome activation. The levels of DNA damage response did not directly correlate with the degree of developmental defects. The early cellular response for DNA damage, γH2AX, is already present at high levels in zygotes that progress normally in development and d

SUBMITTER: Wyck S 

PROVIDER: S-EPMC6192351 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature