Substrate Specificity Analysis of Dihydrofolate/Dihydromethanopterin Reductase Homologs in Methylotrophic α-Proteobacteria.

ABSTRACT: Methane-producing archaea and methylotrophic bacteria use tetrahydromethanopterin (H₄MPT) and/or tetrahydrofolate (H₄F) as coenzymes in one-carbon (C1) transfer pathways. The α-proteobacterium Methylobacterium extorquens AM1 contains a dihydromethanopterin reductase (DmrA) and two annotated dihydrofolate reductases (DfrA and DfrB). DmrA has been shown to catalyze the final step of H₄MPT biosynthesis; however, the functions of DfrA and DfrB have not been examined biochemically. Moreover, sequence alignment (BLAST) searches have recognized scores of proteins that share up to 99% identity with DmrA but are annotated as diacylglycerol kinases (DAGK). In this work, we used bioinformatics and enzyme assays to provide insight into the phylogeny and substrat

SUBMITTER: Burton M 

PROVIDER: S-EPMC6193120 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature