Refinement of Mouse Protocols for the Study of Platelet Thromboembolic Responses In Vivo.

ABSTRACT: Mouse models of thromboembolism are frequently used to investigate platelet function in vivo and, according to European Union (EU) legislation, must be conducted in the context of replacement, refinement and reduction. We have previously developed a refined real-time mouse model of thromboembolism as an alternative to models of thromboembolic mortality which inflict considerable pain and suffering. Real-time monitoring involves infusion of radiolabelled platelets into the circulation of anaesthetized mice, and platelet aggregation is measured as increases in platelet-associated counts in the pulmonary vasculature following injection of platelet agonists. This gives a definitive data set on the tissue localization and extent of platelet activation. We developed an additional, more simplisti

SUBMITTER: Rauzi F 

PROVIDER: S-EPMC6193277 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature