Ontology highlight
ABSTRACT:
SUBMITTER: Banerjee S
PROVIDER: S-EPMC6193456 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Biochemistry 20170124 5
The autocatalytic maturation of the chromophore in green fluorescent protein (GFP) was thought to require the precise positioning of the side chains surrounding it in the core of the protein, many of which are strongly conserved among homologous fluorescent proteins. In this study, we screened for green fluorescence in an exhaustive set of point mutations of seven residues that make up the chromophore microenvironment, excluding R96 and E222 because mutations at these positions have been previou ...[more]