Molecular Imaging of Radiolabeled Bispecific T-Cell Engager ⁸⁹Zr-AMG211 Targeting CEA-Positive Tumors.

ABSTRACT: Purpose: AMG 211, a bispecific T-cell engager (BiTE) antibody construct, targets carcinoembryonic antigen (CEA) and the CD3 epsilon subunit of the human T-cell receptor. AMG 211 was labeled with zirconium-89 (⁸⁹Zr) or fluorescent dye to evaluate the tumor-targeting properties.Experimental Design: ⁸⁹Zr-AMG211 was administered to mice bearing CEA-positive xenograft tumors of LS174T colorectal adenocarcinoma or BT474 breast cancer cells, as well as CEA-negative HL-60 promyelocytic leukemia xenografts. Biodistribution studies with 2- to 10-μg ⁸⁹Zr-AMG211 supplemented with unlabeled AMG 211 up to 500-μg protein dose were performed. A BiTE that does not bind CEA, ⁸⁹Zr-Mec14, served as a negative control. ⁸⁹Zr-AMG211 integrity w

SUBMITTER: Waaijer SJH 

PROVIDER: S-EPMC6193542 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature