Differential profile of protein expression on human keratocytes treated with autologous serum and plasma rich in growth factors (PRGF).

ABSTRACT:

Purpose

The main objective of this study is to compare the protein expression of human keratocytes treated with Plasma rich in growth factors (PRGF) or autologous serum (AS) and previously induced to myofibroblast by TGF-β1 treatment.

Methods

Blood from healthy donor was collected and processed to obtain AS and PRGF eye drops. Blood derivates were aliquoted and stored at -80°C until use. Keratocyte cells were pretreated for 60 minutes with 2.5 ng/ml TGF-β1. After that, cells were treated with PRGF, AS or with TGF-β1 (control). To characterize the proteins deregulated after PRGF and AS treatment, a proteomic approach that combines 1D-SDS-PAGE approach followed by LC-MS/MS was carried out.

Results

Results show a catalogue of key proteins in close contact with a myofibro

SUBMITTER: Anitua E 

PROVIDER: S-EPMC6193583 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature