Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice.

ABSTRACT: Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H₂) and methane (CH₄) sensors. H₂ production was readily detected in C57BL/6J mice and followed a circadian rhythm. H₂ production was increased within 12 hours after first exposure to a lowly-digestible starch diet (LDD) compared to a highly-digestible starch diet (HDD). Marked differences were observed in the faecal microbiota of animals fed the LDD and HDD diets. H₂ was identified as a key variable explaining the variation in microbial communities, with specific taxa (including Bacteroides and Parasutterella) correlating with H₂

SUBMITTER: Fernandez-Calleja JMS 

PROVIDER: S-EPMC6193968 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature