Unique true predicted neoantigens (TPNAs) correlates with anti-tumor immune control in HCC patients.

ABSTRACT:

Background

A novel prediction algorithm is needed for the identification of effective tumor associated mutated neoantigens. Only those with no homology to self wild type antigens are true predicted neoantigens (TPNAs) and can elicit an antitumor T cell response, not attenuated by central tolerance. To this aim, the mutational landscape was evaluated in HCV-associated hepatocellular carcinoma.

Methods

Liver tumor biopsies and adjacent non-tumor liver tissues were obtained from 9 HCV-chronically infected subjects and subjected to RNA-Seq analysis. Mutant peptides were derived from single nucleotide variations and TPNAs were predicted using two prediction servers (e.g. NetTepi and NetMHCstabpan) by comparison with corresponding wild-type sequences, non-related self and pathogen

SUBMITTER: Petrizzo A 

PROVIDER: S-EPMC6194606 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature