Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9.

ABSTRACT:

Background

Targeted DNA integration is widely used in basic research and commercial applications because it eliminates positional effects on transgene expression. Targeted integration in mammalian cells is generally achieved through a double crossover event between the genome and a linear donor containing two homology arms flanking the gene of interest. However, this strategy is generally less efficient at introducing larger DNA fragments. Using the homology-independent NHEJ mechanism has recently been shown to improve efficiency of integrating larger DNA fragments at targeted sites, but integration through this mechanism is direction-independent. Therefore, developing new methods for direction-dependent integration with improved efficiency is desired.

Results

We generated s

SUBMITTER: Liu X 

PROVIDER: S-EPMC6194632 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature