FOS, a Critical Downstream Mediator of PGR and EGF Signaling Necessary for Ovulatory Prostaglandins in the Human Ovary.

ABSTRACT:

Context

Fos null mice failed to ovulate and form a corpus luteum (CL) even when given exogenous gonadotropins, suggesting that ovarian Fos expression is critical for successful ovulation and CL formation. However, little is known about FOS in the human ovary.

Objectives

To determine the expression, regulation, and function of FOS in human periovulatory follicles.

Design/participants

Timed periovulatory follicles were obtained from normally cycling women. Granulosa/lutein cells were collected from in vitro fertilization patients.

Main outcome measures

The in vivo expression after human chorionic gonadotropin (hCG) administration and in vitro regulation of FOS, JUN, JUNB, and JUND was evaluated at the mRNA and protein level. Binding of progesterone receptor (PGR)

SUBMITTER: Choi Y 

PROVIDER: S-EPMC6194814 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature