A microfluidic platform for lifelong high-resolution and high throughput imaging of subtle aging phenotypes in C. elegans.

ABSTRACT: Aging produces a number of changes in the neuronal structure and function throughout a variety of organisms. These aging-induced changes encompass a wide range of phenotypes, from loss of locomotion ability to defective production of synaptic vesicles. C. elegans is one of the primary systems used to elucidate phenotypes associated with aging processes. Conventional aging studies in C. elegans are typically labor-intensive, low-throughput, and incorporate fluorodeoxyuridine (FUdR) as a sterilizing agent to keep the population age-synchronized throughout the assay. However, FUdR exposure induces lifespan extension, and can potentially mask the phenotypes associated with the natural aging process. In addition, studying cellular or subcellular structures requires anesthetics or adhesives to i

SUBMITTER: Saberi-Bosari S 

PROVIDER: S-EPMC6195199 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature