Allele-Specific Isothermal Amplification Method Using Unmodified Self-Stabilizing Competitive Primers.

ABSTRACT: Rapid and specific detection of single nucleotide polymorphisms (SNPs) related to drug resistance in infectious diseases is crucial for accurate prognostics, therapeutics and disease management at point-of-care. Here, we present a novel amplification method and provide universal guidelines for the detection of SNPs at isothermal conditions. This method, called USS-sbLAMP, consists of SNP-based loop-mediated isothermal amplification (sbLAMP) primers and unmodified self-stabilizing (USS) competitive primers that robustly delay or prevent unspecific amplification. Both sets of primers are incorporated into the same reaction mixture, but always targeting different alleles; one set specific to the wild type allele and the other to the mutant allele. The mechanism of action relies on thermodynam

SUBMITTER: Malpartida-Cardenas K 

PROVIDER: S-EPMC6195307 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature