Mutant TP53 modulates metastasis of triple negative breast cancer through adenosine A2b receptor signaling.

ABSTRACT:

Purpose

The identification of genes with synthetic lethality in the context of mutant TP53 is a promising strategy for the treatment of basal-like triple negative breast cancer (TNBC). This study investigated regulators of mutant TP53 (R248Q) in basal-like TNBC and their impact on tumorigenesis.

Experimental design

TNBC cells were analyzed by RNA-seq, and synthetic-lethal shRNA knock-down screening, to identify genes related to the expression of mutant TP53. A tissue microarray of 232 breast cancer samples, that included 66 TNBC cases, was used to assess clinicopathological correlates of tumor protein expression. Functional assays were performed in vitro and in vivo to assess the role of ADORA2B in TNBC.

Results

Transcriptome profiling identified

SUBMITTER: Horigome E 

PROVIDER: S-EPMC6195371 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature