Synthetic Immunotherapeutics against Gram-negative Pathogens.

ABSTRACT: While traditional drug discovery continues to be an important platform for the search of new antibiotics, alternative approaches should also be pursued to complement these efforts. We herein designed a class of molecules that decorate bacterial cell surfaces with the goal of re-engaging components of the immune system toward Escherichia coli and Pseudomonas aeruginosa. More specifically, conjugates were assembled using polymyxin B (an antibiotic that inherently attaches to the surface of Gram-negative pathogens) and antigenic epitopes that recruit antibodies found in human serum. We established that the spacer length played a significant role in hapten display within the bacterial cell surface, a result that was confirmed both experimentally and via molecular dynamics simulations. Most imp

SUBMITTER: Feigman MS 

PROVIDER: S-EPMC6195440 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature