AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules.

ABSTRACT: Cytoskeletal organization is essential for the precise morphogenesis of cells, tissues, and organs. Cytoskeletons, bound to scaffolding proteins, regulate the apical junction complex (AJC), which is composed of tight and adherens junctions, and located at the apical side of epithelial cell sheets. Cingulin is a tight junction-associated protein that binds to both actin filaments and microtubules. However, how cingulin binds to microtubules and whether cingulin can bind to actin and microtubules simultaneously are unclear. Here we examined the mechanisms behind cingulin's cytoskeleton-binding properties. First, using total internal reflection fluorescence microscopy, we detected cingulin at microtubule cross points. We then found the interdomain interactions in cingulin molecules. Notably,

SUBMITTER: Yano T 

PROVIDER: S-EPMC6195624 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature