RNA expression profile of calcified bicuspid, tricuspid, and normal human aortic valves by RNA sequencing.

ABSTRACT: The molecular mechanisms leading to premature development of aortic valve stenosis (AS) in individuals with a bicuspid aortic valve are unknown. The objective of this study was to identify genes differentially expressed between calcified bicuspid aortic valves (BAVc) and tricuspid valves with (TAVc) and without (TAVn) AS using RNA sequencing (RNA-Seq). We collected 10 human BAVc and nine TAVc from men who underwent primary aortic valve replacement. Eight TAVn were obtained from men who underwent heart transplantation. mRNA levels were measured by RNA-Seq and compared between valve groups. Two genes were upregulated, and none were downregulated in BAVc compared with TAVc, suggesting a similar gene expression response to AS in individuals with bicuspid and tricuspid valves. There were 462 ge

SUBMITTER: Guauque-Olarte S 

PROVIDER: S-EPMC6195654 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature