MicroRNA-30 modulates metabolic inflammation by regulating Notch signaling in adipose tissue macrophages.

ABSTRACT:

Background/objectives

Obesity is a pandemic disorder that is characterized by accumulation of adipose tissue and chronic low-grade inflammation that is driven primarily by adipose tissue macrophages (ATMs). While ATM polarization from pro-(M1) to anti-(M2) inflammatory phenotype influences insulin sensitivity and energy expenditure, the mechanisms of such a switch are unclear. In the current study, we identified epigenetic pathways including microRNAs (miR) in ATMs that regulate obesity-induced inflammation.

Subjects/methods

Male C57BL/6J mice were fed normal chow diet (NCD) or high-fat diet (HFD) for 16 weeks to develop lean and diet-induced obese mice, respectively. Transcriptome microarrays, microRNA microarrays, and MeDIP-Seq were performed on ATMs isolated from visceral

SUBMITTER: Miranda K 

PROVIDER: S-EPMC6195825 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature