Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED.

ABSTRACT: Reproducible and stable transgene expression is an important goal in both basic research and biotechnology, with each application demanding a range of transgene expression. Problems in achieving stable transgene expression include multi-copy transgene silencing, chromosome-position effects, and loss of expression during long-term culture, induced cell quiescence, and/or cell differentiation. Previously, we described the "BAC TG-EMBED" method for copy-number dependent, chromosome position-independent expression of embedded transgenes within a BAC containing ~170 kb of the mouse Dhfr locus. Here we demonstrate wider applicability of the method by identifying a BAC and promoter combination that drives reproducible, copy-number dependent, position-independent transgene expression even after in

SUBMITTER: Chaturvedi P 

PROVIDER: S-EPMC6195848 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature