Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus.

ABSTRACT: Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB_1-3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLH_E44G. These carried the sequence of the fusion protein POLH-Δ3AB_1-3 with an additional copy in cis of polh or polh _E44G , respectively, under p10 promoter. Our results show that both viruses expressed POLH-Δ3AB_1-3, which was detected by western blot in purified rOBs with anti-POLH and an

SUBMITTER: Diez M 

PROVIDER: S-EPMC6195870 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature