Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups.

ABSTRACT: A forensic validation study of the Early Access Huaxia™ Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy test revealed the genotyping ability; while consistent results were obtained when comparing the kit with other commercially available systems. In addition, the whole PCR amplification can finish within approximately 45 min, making the system suitable for fast-detection. However, only partial profiles may be obtained with challenging samples, includ

SUBMITTER: Sheng X 

PROVIDER: S-EPMC6197138 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature