Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation.

ABSTRACT:

Background

Despite compelling data describing pro-regenerative effects of all-trans retinoic acid (ATRA) in pre-clinical models of chronic obstructive pulmonary disease (COPD), clinical trials using retinoids for emphysema patients have failed. Crucial information about the specific role of RA signaling in adult rodent and human lung epithelial progenitor cells is largely missing.

Methods

Adult lung organoid cultures were generated from isolated primary mouse and human lung epithelial cells, and incubated with pharmacological pathway modulators and recombinant proteins. Organoid number and size were measured, and differentiation was assessed with quantitative immunofluorescence and gene expression analyses.

Findings

We unexpectedly found that ATRA decreased lung organ

SUBMITTER: Ng-Blichfeldt JP 

PROVIDER: S-EPMC6197151 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature