L-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism.

ABSTRACT:

Objective

AMP activated protein kinase (AMPK) is recognized as an important nutrient sensor contributing to regulation of cellular, tissue, and systemic metabolism. We aimed to identify specific amino acids which could modulate AMPK and determine effects on cellular and systemic metabolism.

Methods

We performed an unbiased amino acid screen to identify activators of AMPK. Detailed analysis of cellular signaling and metabolism was performed in cultured hepatoma cells, and in vivo glucose metabolism and metabolomic patterns were assessed in both chow-fed mice and mice made obese by high-fat diet feeding.

Results

Alanine acutely activates AMP kinase in both cultured hepatic cells and in liver from mice treated in vivo with Ala. Oral alanine administration improves system

SUBMITTER: Adachi Y 

PROVIDER: S-EPMC6197624 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature