Simplified identification of disulfide, trisulfide, and thioether pairs with 213 nm UVPD.

ABSTRACT: Disulfide heterogeneity and other non-native crosslinks introduced during therapeutic antibody production and storage could have considerable negative effects on clinical efficacy, but tracking these modifications remains challenging. Analysis must also be carried out cautiously to avoid introduction of disulfide scrambling or reduction, necessitating the use of low pH digestion with less specific proteases. Herein we demonstrate that 213 nm ultraviolet photodissociation streamlines disulfide elucidation through bond-selective dissociation of sulfur-sulfur and carbon-sulfur bonds in combination with less specific backbone dissociation. Importantly, both types of fragmentation can be initiated in a single MS/MS activation stage. In addition to disulfide mapping, it is also shown that thioet

SUBMITTER: Bonner J 

PROVIDER: S-EPMC6197924 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature