Advanced Microfluidic Device Designed for Cyclic Compression of Single Adherent Cells.

ABSTRACT: Cells in our body experience different types of stress including compression, tension, and shear. It has been shown that some cells experience permanent plastic deformation after a mechanical tensile load was removed. However, it was unclear whether cells are plastically deformed after repetitive compressive loading and unloading. There have been few tools available to exert cyclic compression at the single cell level. To address technical challenges found in a previous microfluidic compression device, we developed a new single-cell microfluidic compression device that combines an elastomeric membrane block geometry to ensure a flat contact surface and microcontact printing to confine cell spreading within cell trapping chambers. The design of the block geometry inside the compression cham

SUBMITTER: Ho KKY 

PROVIDER: S-EPMC6198036 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature