Alterations in the oral microbiome in HIV-infected participants after antiretroviral therapy administration are influenced by immune status.

ABSTRACT:

Objective

To characterize the oral bacterial microbiome in HIV-infected participants at baseline and after 24 weeks of EFV/FTC/TDF.

Design

Thirty-five participants co-enrolled in two AIDS Clinical Trials Group (ACTG) studies, A5272 and A5280, with paired saliva samples and complete data sets were assessed.

Methods

Paired saliva samples were evaluated for bacterial microbiome using 16S rDNA PCR followed by Illumina sequencing. Diversity and differential abundance was compared between groups. A random forest classification scheme was used to determine the contribution of parameters in classifying participants' CD4+ T-cell count.

Results

Bacterial communities demonstrated considerable variability both within participants and between timepoints, although they becam

SUBMITTER: Presti RM 

PROVIDER: S-EPMC6198320 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature