A modified method for isolation of human cardiomyocytes to model cardiac diseases.

ABSTRACT:

Background

Cardiomyocytes derived from animals and induced pluripotent stem cells (iPSCs) are two main cellular models to study cardiovascular diseases, however, neither provides precise modeling of the response of mature human cardiomyocytes to disease or stress conditions. Therefore, there are emerging needs for finding an optimized primary human cardiomyocytes isolation method to provide a bona fide cellular model.

Methods and results

Previous established protocols for the isolation of primary human cardiomyocytes are limited in their application due to relatively low cell yield and the requirement of tissue integrity. Here, we developed a novel, simplified method to isolate human cardiomyocytes robustly with improved viability from tissue slicing. Isolated cardiomyocytes

SUBMITTER: Guo GR 

PROVIDER: S-EPMC6198433 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature