DNA replication and repair kinetics of Alu, LINE-1 and satellite III genomic repetitive elements.

ABSTRACT:

Background

Preservation of genome integrity by complete, error-free DNA duplication prior to cell division and by correct DNA damage repair is paramount for the development and maintenance of an organism. This holds true not only for protein-encoding genes, but also it applies to repetitive DNA elements, which make up more than half of the human genome. Here, we focused on the replication and repair kinetics of interspersed and tandem repetitive DNA elements.

Results

We integrated genomic population level data with a single cell immunofluorescence in situ hybridization approach to simultaneously label replication/repair and repetitive DNA elements. We found that: (1) the euchromatic Alu element was replicated during early S-phase; (2) LINE-1, which is associated with AT-rich

SUBMITTER: Natale F 

PROVIDER: S-EPMC6198450 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature