Disrupted circadian clocks and altered tissue mechanics in primary human breast tumours.

ABSTRACT:

Background

Circadian rhythms maintain tissue homeostasis during the 24-h day-night cycle. Cell-autonomous circadian clocks play fundamental roles in cell division, DNA damage responses and metabolism. Circadian disruptions have been proposed as a contributing factor for cancer initiation and progression, although definitive evidence for altered molecular circadian clocks in cancer is still lacking. In this study, we looked at circadian clocks in breast cancer.

Methods

We isolated primary tumours and normal tissues from the same individuals who had developed breast cancer with no metastases. We assessed circadian clocks within primary cells of the patients by lentiviral expression of circadian reporters, and the levels of clock genes in tissues by qPCR. We histologically exam

SUBMITTER: Broadberry E 

PROVIDER: S-EPMC6198506 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature