Improved Detection of Circulating miRNAs in Serum and Plasma Following Rapid Heat/Freeze Cycling.

ABSTRACT:

Background

The measurement of circulating miRNAs has proven to be a powerful biomarker tool for several disease processes. Current protocols for the detection of miRNAs usually involve an RNA extraction step, requiring a substantial volume of patient serum or plasma to obtain sufficient input material.

Objective

Here, we describe a novel methodology that allows detection of a large number of miRNAs from a small volume of serum or plasma without the need for RNA extraction.

Methods

Three μl of serum or plasma was subjected to three cycles of high and low temperatures (heat/freeze cycles) followed by miRNA arrays.

Results

Our results indicate that miRNA detection following this process is highly reproducible when comparing multiple samples from the same subject.

SUBMITTER: Mariner PD 

PROVIDER: S-EPMC6198569 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature