A Metabolic Labeling Strategy for Relative Protein Quantification in Clostridioides difficile.

ABSTRACT: Clostridioides difficile (formerly Clostridium difficile) is a Gram-positive, anaerobe, spore-forming pathogen, which causes drug-induced diseases in hospitals worldwide. A detailed analysis of the proteome may provide new targets for drug development or therapeutic strategies to combat this pathogen. The application of metabolic labeling (ML) would allow for accurate quantification of significant differences in protein abundance, even in the case of very small changes. Additionally, it would be possible to perform more accurate studies of the membrane or surface proteomes, which usually require elaborated sample preparation. Such studies are therefore prone to higher standard deviations during the quantification. The implementation of ML strategies for C. difficile is

SUBMITTER: Trautwein-Schult A 

PROVIDER: S-EPMC6198727 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature