Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded Screen-Printed Electrode.

ABSTRACT:

Abstract

A sensitive and specific immunosensor for the detection of the hormones cortisol and lactate in human or animal biological fluids, such as sweat and saliva, was devised using the label-free electrochemical chronoamperometric technique. By using these fluids instead of blood, the biosensor becomes noninvasive and is less stressful to the end user, who may be a small child or a farm animal. Electroreduced graphene oxide (e-RGO) was used as a synergistic platform for signal amplification and template for bioconjugation for the sensing mechanism on a screen-printed electrode. The cortisol and lactate antibodies were bioconjugated to the e-RGO using covalent carbodiimide chemistry. Label-free electrochemical chronoamperometric detection was used to analyze the response to the d

SUBMITTER: Tuteja SK 

PROVIDER: S-EPMC6199085 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature