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Using ¹ H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.

ABSTRACT: This report describes a cost-effective experimental method for determining an intrinsically disordered protein (IDP) region in a given protein sample. In this area, the most popular (and conventional) means is using the amide (¹ H^N ) NMR signal chemical shift distributed in the range of 7.5-8.5 ppm. For this study, we applied an additional step: analysis of ¹ H^N chemical shift temperature coefficients (¹ H^N -CSTCs) of the signals. We measured ¹ H-¹⁵ N two-dimensional NMR spectra of model IDP samples and ordered samples at four temperatures (288, 293, 298, and 303 K). We derived the ¹ H^N -CSTC threshold deviation, which gives the best correlation of ordered and disordered regions am

SUBMITTER: Okazaki H

PROVIDER: S-EPMC6199157 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Using ¹ H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.

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Using 1 HN amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.

OmicsDI is part of the ELIXIR infrastructure

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Using ¹ H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.