Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression.

ABSTRACT: Integrin β1 is known to be involved in differentiation, migration, proliferation, wound repair, tissue development, and organogenesis. In order to analyze the binding probability between integrin β1 ligand and cluster of differentiation 29 (CD29) receptors, atomic force microscopy (AFM) was used to detect native integrin β1-coupled receptors on the surface of human adipose-derived stem cells (hADSc). The binding probability of integrin β1 ligand-receptor interaction was probed by integrin β1-functionalized tips on hADSc during early chondrogenic differentiation at the two-dimensional cell culture level. Cell morphology and ultrastructure of hADSc were measured by AFM, which demonstrated that long spindled cells became polygonal cells with decreased length/width ratios and increased roughne

SUBMITTER: Yang J 

PROVIDER: S-EPMC6199198 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature