NFATC2 is a novel therapeutic target for colorectal cancer stem cells.

ABSTRACT:

Background

Colorectal cancer stem cells (CRC-SCs) contribute to the initiation and progression of colorectal cancer (CRC). However, the underlying mechanisms for the propagation of CRC-SCs have remained elusive.

Purpose

The objective of this study was to study the role of NFATC2 in maintenance of the stemness in CRC-SCs.

Method

The expression levels of mRNA and protein were determined by qRT-PCR and western-blot, respectively. CRC-SCs were isolated by spheroid formation assay and flowcytometry. The sphere-forming and self-renewal abilities of CRC-SCs were determined by spheroid formation assay. The tumorigenicity of CRC-SCs was determined by cell-derived xenograft model. Gene manipulation was performed by lentivirus-mediated delivery system.

Results

We first fo

SUBMITTER: Lang T 

PROVIDER: S-EPMC6199214 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature