Characterization and improved properties of Glutamine synthetase from Providencia vermicola by site-directed mutagenesis.

ABSTRACT: In this study, a novel gene for Glutamine synthetase was cloned and characterized for its activities and stabilities from a marine bacterium Providencia vermicola (PveGS). A mutant S54A was generated by site directed mutagenesis, which showed significant increase in the activity and stabilities at a wide range of temperatures. The K_m values of PveGS against hydroxylamine, ADP-Na₂ and L-Glutamine were 15.7 ± 1.1, (25.2 ± 1.5) × 10^-5 and 32.6 ± 1.7 mM, and the k_cat were 17.0 ± 0.6, 9.14 ± 0.12 and 30.5 ± 1.0 s^-1 respectively. In-silico-analysis revealed that the replacement of Ser at 54th position with Ala increased the catalytic activity of PveGS. Therefore, catalytic efficiency of mutant S54A had increased by 3.1, 0.89 and 2.9-folds to

SUBMITTER: Zuo W 

PROVIDER: S-EPMC6199252 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature