Stac Proteins Suppress Ca²⁺-Dependent Inactivation of Neuronal l-type Ca²⁺ Channels.

ABSTRACT: Stac protein (named for its SH3- and cysteine-rich domains) was first identified in brain 20 years ago and is currently known to have three isoforms. Stac2, Stac1, and Stac3 transcripts are found at high, modest, and very low levels, respectively, in the cerebellum and forebrain, but their neuronal functions have been little investigated. Here, we tested the effects of Stac proteins on neuronal, high-voltage-activated Ca²⁺ channels. Overexpression of the three Stac isoforms eliminated Ca²⁺-dependent inactivation (CDI) of l-type current in rat neonatal hippocampal neurons (sex unknown), but not CDI of non-l-type current. Using heterologous expression in tsA201 cells (together with β and α₂-δ₁ auxiliary subunits), we found that CDI for Ca_V

SUBMITTER: Polster A 

PROVIDER: S-EPMC6199411 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature