Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome.

ABSTRACT:

Background

Efficient and sustained hematopoietic recovery after hematopoietic stem cell or bone marrow transplantation is supported by paracrine signaling from specific subpopulations of mesenchymal stromal cells (MSCs). Here, we considered whether in vitro mechanopriming of human MSCs could be administered to predictively and significantly improve in vivo hematopoietic recovery after irradiation injury.

Methods

First, we implemented regression modeling to identify eight MSC-secreted proteins that correlated strongly with improved rescue from radiation damage, including hematopoietic recovery, in a murine model of hematopoietic failure. Using these partial least squares regression (PLSR) model parameters, we then predicted recovery potential of MSC populations that were cult

SUBMITTER: Liu FD 

PROVIDER: S-EPMC6199758 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature