Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH).

ABSTRACT:

Background

Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the disease. Fluorescence in situ hybridization (FISH) is a sensitive and robust method that uses sequence-specific, fluorescence-labeled oligonucleotide probes to detect target organisms from the environment. To study this important pathogen, we developed and standardized a FISH technique for the visualization of P. obducens from Impatiens walleriana tissues using a species-specific 24-mer oligonucleotide probe designed to target a reg

SUBMITTER: Salgado-Salazar C 

PROVIDER: S-EPMC6199785 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature