Role of spinal cyclooxygenase-2 and prostaglandin E2 in fentanyl-induced hyperalgesia in rats.

ABSTRACT:

Background

Accumulated evidence suggests that spinal cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) may be implicated in the development of opioid-induced hyperalgesia.

Methods

Rats received subcutaneous fentanyl injections at different doses (20-80 μg kg^-1), four separate times at 15-min intervals. Some rats only received fentanyl (60 μg kg^-1 × 4 doses) with or without surgical incision. Fentanyl-induced hyperalgesia was evaluated via a tail-pressure or paw-withdrawal test. The concentrations of spinal COX-2, EP-1 receptor (EP-1R) mRNA, and PGE2 were measured. The effects of the COX-2 inhibitor, parecoxib (intraperitoneal 10 mg kg^-1), or the EP-1R antagonist, SC51089 (intraperitoneal 100 μg kg^-1), on hyperalgesia and spinal

SUBMITTER: Li QB 

PROVIDER: S-EPMC6200103 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature