Isoflurane disrupts excitatory neurotransmitter dynamics via inhibition of mitochondrial complex I.

ABSTRACT:

Background

The mechanisms of action of volatile anaesthetics are unclear. Volatile anaesthetics selectively inhibit complex I in the mitochondrial respiratory chain. Mice in which the mitochondrial complex I subunit NDUFS4 is knocked out [Ndufs4(KO)] either globally or in glutamatergic neurons are hypersensitive to volatile anaesthetics. The volatile anaesthetic isoflurane selectively decreases the frequency of spontaneous excitatory events in hippocampal slices from Ndufs4(KO) mice.

Methods

Complex I inhibition by isoflurane was assessed with a Clark electrode. Synaptic function was measured by stimulating Schaffer collateral fibres and recording field potentials in the hippocampus CA1 region.

Results

Isoflurane specifically inhibits complex I dependent respiration a

SUBMITTER: Zimin PI 

PROVIDER: S-EPMC6200108 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature