MAPK signaling has stage-dependent osteogenic effects on human adipose-derived stem cells in vitro.

ABSTRACT:

Overview

The use of pro-osteogenic growth factors, such as BMP2, in human adipose-derived stem cell (ASC) osteogenesis is well described. Because these growth factors work via signal transduction pathways, such as the mitogen-activated protein kinase (MAPK) cascade, a study of the relationship between MAPK signaling and ASC osteogenesis was conducted.

Materials and methods

ERK, JNK, and p38MAPK activation were measured in ASCs osteo-induced using either dexamethasone or vitamin D3 and correlated with mineralization. Activation and mineralization were also measured without dexamethasone or using the glucocorticoid, cortisone. The expression of the MAPK phosphatase, MKP1, and its relationship to mineralization was also assessed. The effect of decreasing MAPK activation on mine

SUBMITTER: Tsang EJ 

PROVIDER: S-EPMC6200338 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature