Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis.

ABSTRACT: Fast synaptic inhibition in the nervous system depends on the transmembrane flux of Cl^- ions based on the neuronal Cl^- driving force. Established theories regarding the determinants of Cl^- driving force have recently been questioned. Here, we present biophysical models of Cl^- homeostasis using the pump-leak model. Using numerical and novel analytic solutions, we demonstrate that the Na⁺/K⁺-ATPase, ion conductances, impermeant anions, electrodiffusion, water fluxes and cation-chloride cotransporters (CCCs) play roles in setting the Cl^- driving force. Our models, together with experimental validation, show that while impermeant anions can contribute to setting [Cl^-]_i in neurons, they have a neg

SUBMITTER: Dusterwald KM 

PROVIDER: S-EPMC6200395 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature