Screening Estrogen Receptor Modulators in a Paper-Based Breast Cancer Model.

ABSTRACT: The health risks associated with acute and prolonged exposure to estrogen receptor (ER) modulators has led to a concerted effort to identify and prioritize potential disruptors present in the environment. ER agonists and antagonists are identified with end-point assays, quantifying changes in cellular proliferation or gene transactivation in monolayers of estrogen receptor alpha expressing (ER+) cells upon exposure. While these monolayer cultures can be prepared, dosed, and analyzed in a highly parallelized manner, they are unable to predict the potencies of ER modulators in vivo accurately. Physiologically relevant model systems that better predict tissue- or organ-level responses are needed. To address this need, we describe here a screening platform capable of quantitatively assessing E

SUBMITTER: Whitman NA 

PROVIDER: S-EPMC6200649 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature