Measuring macromolecular size distributions and interactions at high concentrations by sedimentation velocity.

ABSTRACT: In concentrated macromolecular solutions, weak physical interactions control the solution behavior including particle size distribution, aggregation, liquid-liquid phase separation, or crystallization. This is central to many fields ranging from colloid chemistry to cell biology and pharmaceutical protein engineering. Unfortunately, it is very difficult to determine macromolecular assembly states and polydispersity at high concentrations in solution, since all motion is coupled through long-range hydrodynamic, electrostatic, steric, and other interactions, and scattering techniques report on the solution structure when average interparticle distances are comparable to macromolecular dimensions. Here we present a sedimentation velocity technique that, for the first time, can resolve macromo

SUBMITTER: Chaturvedi SK 

PROVIDER: S-EPMC6200768 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature