Subdiffusion of loci and cytoplasmic particles are different in compressed Escherichia coli cells.

ABSTRACT: The complex physical nature of the bacterial intracellular environment remains largely unknown, and has relevance for key biochemical and biological processes of the cell. Although recent work has addressed the role of non-equilibrium sources of activity and crowding, the consequences of mechanical perturbations are relatively less explored. Here we use a microfabricated valve system to track both fluorescently labeled chromosomal loci and cytoplasmic particles in Escherichia coli cells shortly after applying a compressive force, observing the response on time scales that are too sudden to allow for biochemical response from the cell. Cytoplasmic diffusion slows markedly on compression but the exponent governing the growth of the ensemble-averaged mean-squared displacement of cytopl

SUBMITTER: Yu S 

PROVIDER: S-EPMC6200837 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature