H₂O₂ oxidation of cysteine residues in c-Jun N-terminal kinase 2 (JNK2) contributes to redox regulation in human articular chondrocytes.

ABSTRACT: Reactive oxygen species (ROS), in particular H₂O₂, regulate intracellular signaling through reversible oxidation of reactive protein thiols present in a number of kinases and phosphatases. H₂O₂ has been shown to regulate mitogen-activated protein kinase (MAPK) signaling depending on the cellular context. We report here that in human articular chondrocytes, the MAPK family member c-Jun N-terminal kinase 2 (JNK2) is activated by fibronectin fragments and low physiological levels of H₂O₂ and inhibited by oxidation due to elevated levels of H₂O₂ The kinase activity of affinity-purified, phosphorylated JNK2 from cultured chondrocytes was reversibly inhibited by 5-20 μm H₂O₂ Using dimedo

SUBMITTER: Nelson KJ 

PROVIDER: S-EPMC6200941 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature