Cyclic Diguanylate Regulates Virulence Factor Genes via Multiple Riboswitches in <i>Clostridium difficile</i>.

ABSTRACT: The intracellular signaling molecule cyclic diguanylate (c-di-GMP) regulates many processes in bacteria, with a central role in controlling the switch between motile and nonmotile lifestyles. Recent work has shown that in Clostridium difficile (also called Clostridioides difficile), c-di-GMP regulates swimming and surface motility, biofilm formation, toxin production, and intestinal colonization. In this study, we determined the transcriptional regulon of c-di-GMP in C. difficile, employing overexpression of a diguanylate cyclase gene to artificially manipulate intracellular c-di-GMP. Consistent with prior work, c-di-GMP regulated the expression of genes involved in swimming and surface motility. c-di-GMP also affected the expression of multiple genes encoding cell env

SUBMITTER: McKee RW 

PROVIDER: S-EPMC6200980 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature