Transcriptional Heterogeneity of <i>Cryptococcus gattii</i> VGII Compared with Non-VGII Lineages Underpins Key Pathogenicity Pathways.

ABSTRACT: Cryptococcus gattii is a pathogenic yeast of humans and other animals which causes disease predominantly in immunocompetent hosts. Infection begins when aerosolized yeast or spores enter the body, triggering an immune response, including engulfment by macrophages. To understand the early transcriptional signals in both the yeast and its mammalian host, we performed a time-course dual-transcriptome sequencing (RNA-seq) experiment for four lineages of C. gattii (lineages VGI to IV) interacting with mouse macrophages at 1, 3, and 6 h postinfection. Comparisons of in vitro to ex vivo gene expression levels indicated that lineage VGII is transcriptionally divergent from non-VGII lineages, including differential expression of genes involved in capsule synthesis, capsu

SUBMITTER: Farrer RA 

PROVIDER: S-EPMC6200987 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature