Airborne Pseudomonas species in Healthcare Facilities in a Tropical Setting.

ABSTRACT:

Background and objectives

Pseudomonas species are nosocomial pathogens that are capable of colonising moist surfaces. Little is known whether they get airborne. The study was undertaken to 1) characterise Gram-negative bacteria in indoor air of different hospitals; 2) characterise Pseudomonas sp. by phenotypic and genotypic methods; 3) determine homology of study environmental Pseudomonas isolates and correlate with established pathogenic strains' sequences.

Methods

Samples were collected (duplicates) at the time of peak activity, by exposing media-containing plates (blood agar and MacConkey agar) for 30 minutes. Plates were incubated aerobically at 37°C for 24-48 h. Microorganisms were identified by standard microbiological procedures. Polymerase chain reaction targeting Ps

SUBMITTER: Sudharsanam S 

PROVIDER: S-EPMC6201198 | biostudies-literature | 2015 Apr-Jun

REPOSITORIES: biostudies-literature