Determination of binding affinities using hyperpolarized NMR with simultaneous 4-channel detection.

ABSTRACT: Dissolution dynamic nuclear polarization (D-DNP) is a powerful technique to improve NMR sensitivity by a factor of thousands. Combining D-DNP with NMR-based screening enables to mitigate solubility or availability problems of ligands and target proteins in drug discovery as it can lower the concentration requirements into the sub-micromolar range. One of the challenges that D-DNP assisted NMR screening methods face for broad application, however, is a reduced throughput due to additional procedures and time required to create hyperpolarization. These requirements result in a delay of several tens of minutes in-between each NMR measurement. To solve this problem, we have developed a simultaneous 4-channel detection method for hyperpolarized ¹⁹F NMR, which can increase throughput

SUBMITTER: Kim Y 

PROVIDER: S-EPMC6201311 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature